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Overview
Faculty
Majors
Courses
Instrumentation
Opportunities
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| Dr. Daniel Burden
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Associate
Professor, Analytical Chemistry
Phone: (630) 752-5065
Fax: (630) 752-5996
daniel.l.burden@wheaton.edu
- Honors and Research Support
- Student Researchers
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| Education |
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NRC Postdoctoral Fellow, National Institute of Standards and Technology, Gaithersburg, MD, 1998-2000
Ph.D.,
Analytical Chemistry, Indiana University, Bloomington, IN, 1997
B.S., Chemistry & Physics, Taylor University, Upland, IN, 1992
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Professional and Personal Interests
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Single-Molecule
Spectroscopy of Biomolecules. Ultra-sensitive molecular fluorescence techniques provide a powerful way to analyze the complex behavior of biomolecules because distributional outliers can be observed on an individual basis. This can not be done with any classical approach that measures the average of an ensemble. We are currently employing both confocal and wide-field single-molecule fluorescence microscopy to study the structure, function, and behavior of biomolecules in a number of different chemical systems. Applications of the research range from improvements in the biocompatibility of medical implants to new antiviral and cancer fighting drugs.
Opportunities exist for students to pursue research projects in a variety of related areas. Several areas are briefly outlined below.
 Probing
Diffusion in 3D. Confocal microscopy enables a unique 3D view of samples that is not possible with other forms of microscopy. Dr. Burden utilizes confocal fluorescence microscopy to study a variety of biomembrane interfaces. Such interfaces can be used to model the behavior of cell membranes and are employed in the design of various chemical sensors. Probing samples such as the unsupported lipid bilayer shown here allows the diffusive motion of molecular components to be characterized in specified nanometer-scale regions in or around the membrane. In addition, by controlling and measuring the pressure across a membrane, molecular motion is being studied as a function of applied mechanical stress. Click here to see 3D movie.
 Ion Channel Dynamics. Another facet of Dr. Burden's research focuses on the dynamic behavior of channel-forming proteins in lipid membranes. Various ion channels participate in the propagation of nerve impulses and are found in poisonous venoms and cell-killing toxins. Other mechanically sensitive channels within the inner ear facilitate the detection of acoustical vibrations and help provide a sense of balance. Electrical recording techniques allow individual channels to be characterized as ionic current passes through the pore interior. Combining the instrumentation for single-channel electrical recording with single-molecule fluorescence microscopy provides a means for simultaneously interrogating channels with light and electricity in real time. The combined approach creates an information-rich technique for the basic study of channel dynamics, molecular transport, and intercellular communication.
Wide-field Imaging. The microscopy equipment in the Burden lab is also being used to perform high-speed video imaging of single molecules as they diffuse in lipid membranes. Investigators
are using this instrumentation to divulge new insights into the aggregation of the alpha-hemolysin protein. This protein has been identified as a key factor in MRSA infections, a notorious strain of antibiotic-resistant super bugs.
Click on image to view video.
Simulating Single-Molecule Diffusion Dynamics. Dr. Burden’s lab also develops computer simulations of diffusion to provide an ancillary method of interpreting data from single-molecule fluorescence experiments. In this illustration, diffusion is mimicked by causing simulated molecules to move randomly across a flat 2D surface. The circle in the center represents the 1/e^2 boundary of a confocal microscope’s detection region. When fluorescent molecules enter this area, photons are produced and detected as a function of simulated time. A record of individual molecular entries and exits from the detection circle is registered as a plot of photon intensity vs. time.
 Partnering with Local Business. Biomolecular Nanotechnologies and Measurements (BiNano) is a small business that works in conjunction with Wheaton College to provide scientific research and consulting services. It also functions as an incubator to stimulate new investigative ventures involving Wheaton College faculty, staff, and students. The company laboratories are housed on the campus of Wheaton College. The main business office is located at 203 Kellogg Pl., Wheaton, IL. For more information contact Dr. Daniel Burden (630) 752-5065 or Dr. Lisa Burden (630) 752-5693.
After finishing his Ph.D., Dr. Daniel Burden and his wife, Dr. Lisa Burden (a Ph.D. biochemist), took up residence for 2.5 years outside of Washington, D.C. where they both worked for national laboratories. The first Dr. Burden (Lisa) was a postdoctoral researcher at the National Institutes of Health in Bethesda , MD. The second Dr. Burden (Daniel) was a National Research Council Associate at the National Institute of Standards and Technology in Gaithersburg , MD. The Burden's have three children (Hannah, Luke, and Nathan) who were born in 1999 and 2003, and 2006 respectively. Both Dr. Burdens enjoy exercising, watching a variety of sports (football, baseball, and college basketball) and playing music with their children.
| Courses
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- General Chemistry
- Introduction to Analytical Chemistry
- Advanced Analytical Chemistry I
- Advanced Analytical Chemistry II
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Membership in Professional Societies
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- American Chemical Society
- Sigma Xi, Scientific Honor Society
- Biophysical Society
- American Scientific Affiliation
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Research
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Honors and Research Support
- NRC Postdoctoral Fellow, 1998
- Wheaton College Alumni Association, 2000- present
- Camille and Henry Dreyfus Faculty Start up Award, 2000
- ACS-PRF Award, 2001, 2004, 2007
- Research Corporation Award, 2001, 2004
- Camille and Henry Dreyfus Teacher-Scholar Award, 2005
- Indiana University Award for Excellence in Undergraduate Chemical Research,
2005
- NSF-RUI, 2006
- NSF-MRI, 2007
Research Students, 2001-present (Click
names for bios, projects, posters, presentations)
Dan Bailey, Derek
Bailey, Mark
Brown, Joe
Burkett, Josh
Carr, Emily
Chandler,
Wayland
Cheng, Michael
Culbertson, Dan
Dreyer, Dan Fager, Ryan
Himes, Katie
Hubbell,
Brynna
Jones, Jared Kindt, Daniel
Kim, Jonathan Kooiman, Jonathan Lam, Amy Larson, Matt
Lovell, Travis
Lowens, Ashley
Paulson,
Steven
Poppen, Nathan Skinner, Alyssa
Smith, Kelsey Schuster, Dee
Ann Stults, Michael
Madison Taylor, Donna Van Cott,
Emily
Rupp Wiebracht, Josh Williams
| Selected Papers
Published and/or Presented |
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(student co-authors
in bold)
Reiner, J., Jahn, A., Stavis, S., Culbertson, M.J., Vreeland, W., Burden, D.L., Geist, J., Gaitan, M., "Accurate Optical Analysis of Single-Molecule Entrapment in Nanoscale Vesicles," Anal. Chem., 82, 180-188, 2010.
Carr, J., Himes, R.D., Keung, C.H., Burden, D.L., Walhout, P.W., Heterogeneous Translational Dynamics of Rhodamine B in Polyelectrolyte Multilayer Thin Films Observed by Single Molecule Microscopy, " Langmuir, 25, 8330, 2009.
Williams, J.T.B, Culbertson, M.J., Burden, D.L., "Numerical Fluorescence Correlation Spectroscopy - A Unique Opportunity for Advancement in Biotechnological Systems Research, Am. Biotech. Lab., 26, 6, 2008.
Culbertson, M.J., Williams, J.T.B., Cheng, W., Stults, D.A,
Wiebracht, E., Kasianowicz, J.J., Burden, D.L., “Numerical Fluorescence
Correlation Spectroscopy for the Analysis of Molecular Dynamics under
Non-standard Conditions,” Anal. Chem., 79, 4031-4039, 2007.
Culbertson,
M.J ., Burden, D.L., “A Distributed Algorithm for
Multi-tau Autocorrelation,” Rev. Sci. Inst., 78, 044102, 2007.
Chandler, E.L., Smith, A.L., Burden, L.M., Kasianowicz, J.J., Burden,
D.L., “Membrane Surface Dynamics of DNA-Threaded Nanopores Revealed
by Simultaneous Single-Molecule Optical and Ensemble Electrical Recording,” Langmuir,
20, 898-905, 2004.
Burden, D.L., Walhout, P.K., Elliott, J.T, Chandler, E.L., Scharf,
R.G., Culbertson, M.J., Stults, D.A., Rupp, E.L., Poppen, S.D., “Measuring
Diffusion in Nanoscale Environments with Single-Molecule Confocal
Fluorescence Microscopy,” Spectroscopy Letters, 37, 129-149,
2004.
Elliott, J.T., Burden, D.L., Woodward, J., Seghal, A., Douglas, J., “Phospholipid
Monolayers Supported on Spun Cast Polystyrene Films,” Langmuir,
19, 2275-2283, 2003.
Burden, D.L., Kasianowicz, J.J., "Diffusion Bias and Photophysical
Dynamics of Single Molecules in Unsupported Lipid Bilayer Membranes
Probed with Confocal Microscopy," J. Phys. Chem. B, 104, 6103
, 2000.
Burden, D.L., Kasianowicz, J.J., "Interrogating Unsupported
Lipid Bilayers with Scanning Confocal Single-Molecule Fluorescence
Microscopy," Proc. SPIE, 3922, 27, 2000.
Leach, A.M.; Burden, D.L.; Hieftje, G.M., "Radioluminescence
Detector for the Flow Injection Determination of Phosphorous as Vanadomolybdophosphoric
Acid," Anal. Chim. Acta., 402, 267, 1999.
Kasianowicz, J.J.; Burden, D.L.; Han, L.C.; Cheley, S.; Bayley, H., "Genetically
Engineered Metal Ion Binding Sites on the Outside of a Channel's Transmembrane
b-Barrel," Biophys. J., 76, 837, 1999.
Burden, D.L.; Hieftje, G.M., "Comparison of Digital Correlation
Techniques in Time-resolved Fluorometry using a Radionuclide-scintillation
Excitation Source," Rev. Sci. Instrum., 70, 50, 1999.
ASBMB, San Diego, CA, April 5-9, 2008; Taylor, M.M., Bailey, D.J., Paulson, A.R., Keranen-Burden, L.M., Burden, D.L.; “Electrophysiological characterization of deriva-tized alpha-hemolysin (aHL) for single-molecule fluorescence stud-ies in planar lipid membranes.”
ASBMB, San Diego, CA, April 5-9, 2008; Paulson, A.R., Jones, B.H., Bailey, D.J., Hubbell, K.L., Burden, D.L., Keranen-Burden, L.M.; “ Recombinant expression, purification, and construction of fluorescently labeled alpha-hemolysin (aHL) for single-molecule optical studies.”
Biophysical Society, Baltimore, MD, March 3-7, 2007; Burden, D.L., Culbertson,
M. J., "Implementing Numerical Fluorescence Correlation
Spectroscopy Under Non-Standard Measurement Conditions to Accurately
Assess Molecular Dynamics."
PittCon, Chicago, IL, Feb 25-March 1,2007, Burden, D. L., Culbertson,
M. J., Williams, J. T., “Numerical Fluorescence Correlation
Spectroscopy for the Analysis of Complex Biomolecular Dynamics.”
PittCon, Orlando, FL, Feb 27-March 4, 2005, Kim, D., Cheng,
W., Burden
D.L., “Stabilizing Lipid Membrane Sensing Platforms with a
Cytoskeletal Network.”
PittCon, Orlando, FL, Feb 27-March 4, 2005, Burden, D.L., Chandler,
E.L., Smith, A.L., Burden, L.M., Kasianowicz, J.J., “Simultaneous
Single-Molecule Optical and Electrical Recording of DNA/Protein
Analytes Interacting with Nanopores in Membrane Bilayers.”
American Chemical Society, GLRM, Peoria, IL, Oct. 17-20, 2004, Cheng,
W., Kim, D., Burden D.L., “Stabilizing Planar Lipid Bilayer
Membranes.”
American Chemical Society, GLRM, Peoria, IL, Oct. 17-20, 2004, Walhout,
P.K., Burden, D.L., “Single-Molecule Diffusion Measurements
in Nanoscale Environments.”
American Chemical Society, GLRM, Peoria, IL, Oct. 17-20, 2004,
Burden, D.L., Chandler, E.L., Smith, A.L., Burden, L.M., Kasianowicz,
J.J., Culbertson, M.J., “Probing Lipid and Nanopore Dynamics
in Biomembrane Systems with Single-Molecule Microscopy and Electrical
Recording.”
Federation of Analytical Chemistry and Spectroscopy Society (FACSS),
Portland, OR, Oct. 3-7, 2004, Chandler, E.L., Smith, A.L., Burden,
L.M., Kasianowicz, J.J., Burden, D.L. “Simultaneous Single-Molecule
Optical and Electrical Recording of DNA Interactions with Nanopores
and Lipid membranes”
Federation of Analytical Chemistry and Spectroscopy Society (FACSS),
Ft. Lauderdale, FL, Oct. 19-23, 2003; Burden, D.L., Culbertson,
M.J., Elliott, J.T., Woodward, J., Stults, D.A., Poppen, S.D.,
Rupp, E.R “Characterizing
Diffusion on Biomimetic Surfaces with Single-Molecule Fluorescence
Microscopy and Single-Event Duration Histograms.”
Federation of Analytical Chemistry and Spectroscopy Society (FACSS),
Ft. Lauderdale, FL, Oct. 19-23, 2003; Culbertson, M.J., Burden,
D.L., “Chemometric
Approaches to Quantifying Single-Molecule Surface Diffusion with
the Single Event Duration Histogram.”
American Chemical Society, New Orleans, LA, March 23-27, 2003; Brown,
M.C., Burden, D.L., “Investigating Single Molecule Fluorescence
Dynamics on Surfaces.”
American Chemical Society, New Orleans, LA, March 23-27, 2003; Stults,
D.A., Rupp, E.L., Burden, D.L., “Simulating and Analyzing
Molecular Movement in Lipid Membranes.”
CUR (Council on Undergraduate Research), 9th National Conference,
Connecticut College, June 19-23, 2002; Burden, D.L., Chandler,
E.L., Lovell, M.E., Poppen, S.D., Rupp, E.L., “Research in Single
Molecule Dynamics with Undergraduates.”
Faculty continued: Dr. Larry Funck
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